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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
31/05/2016 |
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Data da última atualização: |
08/11/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KUNERT-FILHO, H. C.; CIBULSKI, S. P.; FINKLER, F.; GRASSOTTI, T. T.; JAENISCH, F. R. F.; BRITO, K. C. T. de; CARVALHO, D.; LOVATO, M.; BRITO, B. G. de. |
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Afiliação: |
HIRAN C. KUNERT-FILHO, Instituto de Pesquisas Veterinárias Desidério Finamor; SAMUEL PAULO CIBULSKI, Instituto de Pesquisas Veterinárias Desidério Finamor; FABRINE FINKLER, Instituto de Pesquisas Veterinárias Desidério Finamor; TIELA T. GRASSOTTI, Instituto de Pesquisas Veterinárias Desidério Finamor; FATIMA REGINA FERREIRA JAENISCH, CNPSA; KELLY CRISTINA TAGLIARI DE BRITO, Instituto de Pesquisas Veterinárias Desidério Finamor; DAIANE CARVALHO, Instituto de Pesquisas Veterinárias Desidério Finamor; MARISTELA LOVATO, UFSM; BENITO GUIMARÃES DE BRITO, Instituto de Pesquisas Veterinárias Desidério Finamor. |
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Título: |
First phylogenetic analysis of Avipoxvirus (APV) in Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 5, p. 357-362, maio. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from
turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in
a turkey housing system. The birds displaying characteristic pox lesions which were observed
on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly
chosen, euthanized and necropsied. Tissues samples were submitted for histopathological
analysis and total DNA was further extracted, amplified by conventional PCR, sequenced
and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b
core protein gene sequence. The histological analysis revealed dermal inflammatory process,
granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene
was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence
identity among the samples, and the sequences were deposited in GenBank®. The
four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of
avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus
isolation, PCR and sequencing includinga large number of specimens from the Brazilian
turkey production must be conducted due to the hazardous risk that poxvirus infections
may cause to the Brazilian poultry production scenario, given that Brazil?s turkey production
attracts attention due to its economic importance worldwide. Our findings point to
the need to identify the prevalence of APV in Brazilian turkey production, to perform risk
assessment studies and continued surveillance of APV infections in both wild and commercial
avian species. MenosAbstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from
turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in
a turkey housing system. The birds displaying characteristic pox lesions which were observed
on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly
chosen, euthanized and necropsied. Tissues samples were submitted for histopathological
analysis and total DNA was further extracted, amplified by conventional PCR, sequenced
and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b
core protein gene sequence. The histological analysis revealed dermal inflammatory process,
granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene
was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence
identity among the samples, and the sequences were deposited in GenBank®. The
four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of
avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus
isolation, PCR and sequencing includinga large number of specimens from the Brazilian
turkey production must be conducted due to the hazardous risk that poxvirus infections
may cause to the Brazilian poultry production scenario, given that Brazil?s turkey production
attracts attention due to its economic importance worldwide. Our findings point ... Mostrar Tudo |
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Palavras-Chave: |
Análise filogenética; APV; Brasil; PCR; Turkey. |
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Thesagro: |
Avicultura; Peru; Virologia. |
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Thesaurus Nal: |
Acyrthosiphon pisum virus; Avipoxvirus; Brazil; Phylogeny; Poxviridae; Turkeys. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143553/1/First-phylogenetic.pdf
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Marc: |
LEADER 02694naa a2200385 a 4500
001 2047236
005 2016-11-08
008 2016 bl uuuu u00u1 u #d
100 1 $aKUNERT-FILHO, H. C.
245 $aFirst phylogenetic analysis of Avipoxvirus (APV) in Brazil.
260 $c2016
520 $aAbstract: This study represents the first phylogenetic analysis of avian poxvirus recovered from turkeys in Brazil. The clinical disorders related to fowlpox herein described occurred in a turkey housing system. The birds displaying characteristic pox lesions which were observed on the neck, eyelids and beak of the turkeys. Four affected turkeys were randomly chosen, euthanized and necropsied. Tissues samples were submitted for histopathological analysis and total DNA was further extracted, amplified by conventional PCR, sequenced and phylogenetically analyzed. Avian poxviruses specific PCR was performed based on P4b core protein gene sequence. The histological analysis revealed dermal inflammatory process, granulation tissue, hyperplasia of epithelial cells and inclusion bodies. The P4b gene was detected in all samples. Sequencing revealed a 100% nucleotide and amino acid sequence identity among the samples, and the sequences were deposited in GenBank®. The four Avian poxviruses fragments sequenced in this study clustered along the A1 clade of avipoxviruses, and were classified as Avipoxvirus (APV). Additional studies, such as virus isolation, PCR and sequencing includinga large number of specimens from the Brazilian turkey production must be conducted due to the hazardous risk that poxvirus infections may cause to the Brazilian poultry production scenario, given that Brazil?s turkey production attracts attention due to its economic importance worldwide. Our findings point to the need to identify the prevalence of APV in Brazilian turkey production, to perform risk assessment studies and continued surveillance of APV infections in both wild and commercial avian species.
650 $aAcyrthosiphon pisum virus
650 $aAvipoxvirus
650 $aBrazil
650 $aPhylogeny
650 $aPoxviridae
650 $aTurkeys
650 $aAvicultura
650 $aPeru
650 $aVirologia
653 $aAnálise filogenética
653 $aAPV
653 $aBrasil
653 $aPCR
653 $aTurkey
700 1 $aCIBULSKI, S. P.
700 1 $aFINKLER, F.
700 1 $aGRASSOTTI, T. T.
700 1 $aJAENISCH, F. R. F.
700 1 $aBRITO, K. C. T. de
700 1 $aCARVALHO, D.
700 1 $aLOVATO, M.
700 1 $aBRITO, B. G. de
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 36, n. 5, p. 357-362, maio. 2016.
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Embrapa Suínos e Aves (CNPSA) |
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